IN VITRO ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF VITISVINIFERA EXTRACTS ON HUMAN BREAST ADENOCARCINOMA CELLS-MCF-7

In vitro studies were carried out to evaluate antioxidant and cytotoxic potential of methanolic, ethanolic and aqueous extracts from
seeds of Vitis vinifera against human breast cancer cells (MCF-7).Screening of these extracts was done using DPPH assay, Reducing
power assay and MTT assay for its antioxidant and cytotoxic activity respectively. All the extracts showed antioxidant activity by
increasing absorbance with concentration (50-400µg/ml ppm) by reducing power method. Among all the extracts tested for their
reducing abilities methanolic and aqueous extract of Vitis vinifera seed demonstrated significant reducing power as shown by the
increasing optical density at 700nm by the potassium ferricyanide reduction method. Vitis vinifera methanolic extract and ethanolic
extract showed 70.08, 74.49 and 34.66, 66.57 inhibition at 50, 100µg/ml (ppm) respectively as measured by DPPH radical scavenging
assay. The methanolic and aqueous extract of vitis vinifera seed showed IC50 220.00±17.32µg/ml and 550.00±0.2 µg/ml in MTT
assay for MCF-7 cells and thus showed significant cytotoxic activity. From results it was observed that methanolic and aqueous
extract of vitis vinifera may be promising alternative to synthetic substances as natural compound with high antiproliferative activities
and to be useful in cancer treatment and prevention.