SYNTHESIS AND ANTITUMOR ACYIVITY OF NEW 2-(PYRIDIN-4-YL) THIAZOLO [3, 2-B][1,2,4]TRIAZOL-6(5H)-ONE DERIVATIVES

The synthesis of proposed biheterocyclic system was carried by using Isoniazide as starting material, which was converted in to 5-
(pyridin-4-yl)-4H-1,2,4-triazole-3-thiol (INHTZ) via 2-isonicotinoylhydrazine-1-carbothioamide by reacting with potassium
thiocyanate and followed cyclisation with sodium hydroxide as catalyst upon refluxing in alcoholic medium. The 5-(pyridin-4-yl)-4H-
1,2,4-triazole-3-thiol (INHTZ) was converted into 2-(pyridin-4-yl) thiazolo [3, 2-b][1,2,4]triazol-6(5h)-one (TrTh 1-12) by one step
multi-component reaction (via thioaceticacids followed by cyclisation in to 2-(pyridin-4-yl)thiazolo[3,2-b][1,2,4]triazol-6(5H)-ones
which were not isolated) by refluxing for 8 hrs with chloro acetic acid in the presence of sodium acetate, acetic anhydride and glacial
acetic acid in alcoholic medium. The synthesized compounds were characterized by physicochemical and analytical data and
evaluated for in-vitro anticancer activity against the two cell lines, some of the derivatives showed excellent in-vitro cytotoxicity.