Plant hairy roots offer a novel and sustainable tissue-based system that preserves the multiple specialized cell types which are
important in maintaining better consistency in the synthesis of bioactive secondary molecules. Bhringraj belonging to family
Asteraceae, Eclipta alba (L.) Hassk. was transformed by Agrobacterium rhizogens. Bacterial gene transfer was confirmed using these
markers. The rol B genes were detected using PCR analysis. This paper will review the state of art report on improving the production
of secondary metabolites in tissue culture in different plant species with special reference to Eclipta alba.