Aquatic organisms are susceptible to contamination of organic and inorganic discharge. Physico-chemical parameters as Temperature,
pH, Dissolved Oxygen, Free Carbon dioxide, Hardness, Chemical Oxygen Demand, Total Solids, Total Dissolved Solids, Suspended
Solids, Chloride, Alkalinity, Nitrate, Phosphate and contamination of heavy metals like Fe+
and K+ has direct or indirect impact
over the metabolic content of targeted cells. Altered physico-chemical parameters influences gametogenic mechanism of crab
Barytelphusa cunicularis. Testicular cells of Barytelphusa cunicularis showed hypertrophy but has reduced content of gametogenic
cells leading to infertility. The obtained results were discussed in relation to influence of physico-chemical parameter over
reproduction of crab Barytelphusa cunicularis.