COMPARATIVE EVALUATION OF ANTIMICROBIAL EFFICACY OF DIFFERENT ROOT CANAL SEALERS AGAINST THE MICROORGANISM ENTEROCOCCUS FAECALIS IN AN EX VIVO INFECTED ROOT CANAL MODEL BY USING COLONY FORMING UNIT

Introduction: Root canal sealers help to minimize leakage provides antimicrobial activity to reduce the possibility of residual bacteria
and to resolve periapical lesion. So the aim of this exvivo study was to compare the antimicrobial activity of five different root canal
sealers against E .faecalis in an infected root canal model by using Colony Forming Unit (CFU).
Materials and Methods: One hundred and twenty single rooted human mandibular premolars were selected. Contamination of the
dentine specimens were carried out for 21 days at 37ºC. The samples (120) were divided into six groups, Group1 –Tubliseal, Group 2-
Apexitplus, Group 3- Fillapex, Group 4- AH Plus, Group 5- Roekoseal, Group 6- Positive control. Five groups after the incubation
with the microorganisms E. faecalis was coated with different root canal sealers and obturated using F3 protaper guttapercha point
(Dentsply Tulsa dental, USA). Then, approximately 3 mm from the apical portion of the samples were resected horizontally and
dentine blocks were obtained (11mm). The root canal filling was removed using a size 2(ISO size90) and 3(ISO size 110) pesso
reamers, and the root canals were then enlarged using size 4(ISO size 130mm) to obtain the dentinal shavings from each of the sample
and analyzed for CFU.
Results: The CFU analysis showed highest antibacterial activity with AH Plus (13700 CFU/ml)and least byRoekoseal(33750
CFU/ml)against E. faecalis.
Conclusion: It can be concluded that the five different root canal sealers that were used in this study exhibited antimicrobial activity.
The maximum antimicrobial activity was achieved AH Plus and Tubliseal. The least antimicrobial activity was achieved with
Roekoseal.